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用途
sufficient for 200 tests
品質等級
包裝
pkg of 1 kit
技術
flow cytometry: suitable
應用
cell analysis
detection
檢測方法
fluorometric
運輸包裝
wet ice
儲存溫度
2-8°C
特點和優勢
相比于TUNEL等基于DNA的检测方法，该方法能更早发现细胞凋亡进程。
- 快速标记细胞。只需10分钟即可完成细胞染色。
- 无需细胞固定或预处理，缩短检测时间并允许在后续研究中继续使用细胞。
- 附带6-羧基荧光素二醋酸盐二级染料，可从活细胞和坏死细胞中区分凋亡细胞。
原則
Sigma的Annexin V-Cy3试剂盒可以配合荧光显微镜来检测凋亡细胞上结合的膜联蛋白V。该试剂盒采用Cy3.18作为偶联膜联蛋白的荧光染料。通过显微镜检测发现，Cy3.18的荧光亮度比FITC偶联物更高。该试剂盒包含非荧光化合物6-羧基荧光素二醋酸盐 (6-CFDA)，后者可以进入到细胞并被活细胞中的酯酶水解，形成荧光化合物6-羧基荧光素，从而证明细胞是否存活。结合荧光显微镜，就可以区分出早期凋亡细胞（膜联蛋白V阳性，6-CFDA阳性）、坏死细胞（膜联蛋白V阳性，6-CFDA阴性）和活细胞（膜联蛋白V阴性，6-CFDA阳性）。
法律資訊
Cy3 is a trademark of Cytiva
仅试剂盒组分
产品编号
说明
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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