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Merck

A8275

Sigma-Aldrich

抗-兔IgG(全分子)-过氧化物酶 山羊抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

生物来源

goat

偶联物

peroxidase conjugate

抗体形式

IgG fraction of antiserum

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

技术

direct ELISA: 1:10,000

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

IgG是一种单体,是一种在血液中循环的抗体,可以协助吞噬破坏外来微生物。它在抗体依赖性细胞介导的细胞毒性中发挥重要作用,并且还与II型和III型超敏反应有关。抗兔IgG(全分子)过氧化物酶偶联抗体可用于pAb-ELISA。兔抗IgG抗体与过氧化物酶(在TBS、0.5% BSA中以1:2500稀释)可用于蛋白质印迹。该抗体还用作间接ELISA法进行蛤蚌毒素分析的检测器。山羊抗兔IgG过氧化物酶缀合物与所有兔免疫球蛋白发生特异性反应。

免疫原

纯化兔 IgG。

应用

抗兔 IgG(全分子)过氧化物酶结合物抗体用于蛋白印迹法、间接 ELISA、免疫印迹和免疫检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
用HRP标记的山羊抗兔IgG作为二抗,对小鼠肠、肝和脾的冰冻切片 (10um) 进行免疫组化分析。在与二抗孵育之前,用MeOH/过氧化氢30%的混合物处理切片,以阻断内源过氧化物酶。

外形

0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)的溶液。

制备说明

按照Wilson, M.B.,和Nakane, P.K.在由阿姆斯特丹Elsevier/北荷兰生物医学出版社出版的免疫荧光及相关染色技术(1978)一书中所描述的高碘酸盐法而制备。

免责声明

除非我们的目录或随附于产品的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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