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A6103

Sigma-Aldrich

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

别名:

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

EC 号:
232-994-9
UNSPSC代码:
12352202
NACRES:
NA.77

产品名称

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

重组

expressed in Nicotiana (tobacco)

质量水平

300

描述

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

产品线

BioUltra

方案

≥98% (SDS-PAGE)

表单

powder

比活

≥5 TIU/mg protein

分子量

~_6.5 kDa

技术

inhibition assay: suitable

溶解性

0.85% sodium chloride: 5 mg/mL

UniProt登记号

P00974

储存温度

2-8°C

基因信息

cow ... PTI(404172)

相关类别

单位定义

一个胰蛋白酶抑制剂单位 (TIU) 将使 2 个胰蛋白酶单位的活性下降 50%，其中 1 个胰蛋白酶单位将在 25°C pH 7.8 下每分钟水解 1.0 μmol 的 N-α-苯甲酰-DL-精氨酸对硝基苯胺 (BAPNA)。

制备说明

不含动物源性成分或杂质，通过在非转基因烟草属植物中繁殖的 RNA (+) 链烟草花叶病毒载体中瞬时表达抑肽酶信息生产。抑肽酶是天然的牛抑肽酶的重组形式，传统上通过分级沉淀、凝胶过滤和离子交换色谱法从牛肺中分离得到。与动物来源的抑肽酶不同，本品采用专有方法从植物组织中分离纯化。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH9755
SLCG0626
SLBW2421
SLBQ7398V
SLBJ8427V

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商品

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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实验方案

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

抑肽酶酶活测定

抑肽酶是一种牛胰蛋白酶抑制剂。在测定抑肽酶活性时，该测定使用了在405 nm下的连续分光光度法进行测定。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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