跳转至内容
Merck

A2909

Sigma-Aldrich

兔IgG-琼脂糖

saline suspension

别名:

IgG agarose beads

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

共軛

agarose conjugate

品質等級

形狀

saline suspension

標籤範圍

≥5 mg per mL

技術

immunoprecipitation (IP): suitable

基質

cross-linked 4% beaded agarose

基質活化

cyanogen bromide

基質墊片

1 atom

儲存溫度

2-8°C

一般說明

IgG是调节几种免疫应答的糖蛋白抗体。IgG-琼脂糖是一种免疫吸附剂,可用于纯化抗体,去除物种特异性交叉反应抗体,或从抗血清制剂中去除污染抗体。一般来说,可以使用相等树脂体积的IgG-琼脂糖从抗血清制剂中除去交叉反应抗体。然而,树脂与抗血清的比例将随个体应用而变化。免疫球蛋白G(IgG)是免疫球蛋白家族的一部分,并且是一种广泛表达的血清抗体。它的恒定(C)区域包含一条γ 重链。IgG的单体150kDa结构分别由两条相同的重链和两条相同的轻链组成,其分子量分别为50kDa和25kDa。该抗体的一级结构还含有二硫键,可用于连接两条重链、连接重链和轻链、以及链内的连接。IgG可进一步细分为四类,即具有不同重链(γ 1、γ 2、γ 3和γ 4)的IgG1、IgG2、IgG3和IgG4。

應用

兔IgG-琼脂糖已被用于免疫沉淀 和亲和纯化检测
将与琼脂糖珠交联的兔IgG抗体(20 μl/ml)用于从293T细胞的全细胞提取物中分离RAT标记的蛋白。将交联至琼脂糖珠的兔IgG用于从哺乳动物全细胞提取物中纯化标记蛋白质(0.5至1.0mg总蛋白质结合至10 μl珠子上)。

外觀

其悬浮于含有防腐剂的0.5M NaCl中。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Emmanuel Vanrobays et al.
RNA (New York, N.Y.), 14(10), 2061-2073 (2008-08-30)
Eukaryotic ribosome synthesis is a highly dynamic process that involves the transient association of scores of trans-acting factors to nascent pre-ribosomes. Many ribosome synthesis factors are nucleocytoplasmic shuttling proteins that engage the assembly pathway at early nucleolar stages and escort
Lingjuan Shan et al.
Journal of genetics and genomics = Yi chuan xue bao, 44(2), 95-106 (2017-02-14)
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and
A Colley et al.
Molecular and cellular biology, 20(19), 7238-7246 (2000-09-13)
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here we report the analysis of two novel DEAH-box RNA helicases, Dhr1p
David Atencio et al.
G3 (Bethesda, Md.), 4(3), 523-537 (2014-01-29)
The Ess1 prolyl isomerase from Saccharomyces cerevisiae and its human ortholog, Pin1, play critical roles in transcription by regulating RNA polymerase II. In human cells, Pin1 also regulates a variety of signaling proteins, and Pin1 misexpression is linked to several
Antibody structure, instability, and formulation
Wang W, et al.
Journal of Pharmaceutical Sciences (2007)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门