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Merck

A1603

Sigma-Aldrich

藻酸盐裂解酶

powder, ≥10,000 units/g solid

别名:

poly-beta-D-mannuronate lyase

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

powder

比活性

≥10,000 units/g solid

儲存溫度

2-8°C

一般說明

藻酸盐裂解酶存在于几种海藻、海洋软体动物以及多种海洋和陆地细菌中。

應用

藻酸盐裂解酶可用于分解藻酸盐或海藻酸,也可用于降低粘度。可用于卵泡分离、包封和培养过程中
藻酸盐裂解酶已被用于:
  • 酶的预处理
  • 去封装细胞
  • 消化假单胞菌外多糖,以促进电穿孔

生化/生理作用

藻酸盐裂解酶可积极参与酸性多糖的糖化,以生产生物乙醇。
可将藻酸盐或海藻酸分解成小分子并降低粘度。

單位定義

在37℃、pH 6.3条件下,一单位酶可使每毫升藻酸钠溶液的A235nm每分钟增加1.0

準備報告

将0.15 ml酶溶液(1 un/ml)加到4.5 ml 0.1%藻酸钠(pH 6.3)中。 37℃孵育30分钟。加入4.65 ml 0.1 N NaOH可终止反应。

其他說明

想查看有关 复合碳水化合物分析酶 的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Elizabeth M Parrish et al.
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Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
Adam J Wargacki et al.
Science (New York, N.Y.), 335(6066), 308-313 (2012-01-24)
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio
Lena C E Lundqvist et al.
Carbohydrate research, 352, 44-50 (2012-03-21)
The gene coding for an alginate lyase from the marine bacteria Pseudomonas alginovora X017 was cloned and heterologously expressed in Escherichia coli strains. The protein was produced in inclusion bodies and the active form was obtained by applying a refolding
Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells
Alemayehu D, et al.
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