A0924

丙烯酰胺/N,N'-亚甲基双丙烯酰胺配制试剂

BioReagent, for molecular biology, 49:1 (ratio)

• 别名: Acrylamide/Bisacrylamide, 49:1 (2.0% crosslinker), Polyacrylamide solution
• MDL编号: MFCD00080848
• UNSPSC代码: 41105319
• PubChem化学物质编号: 329770639
• NACRES: NA.25

属性

• 等级: Molecular Biology; for molecular biology
• 产品线: BioReagent
• 表单: powder
• 投料比: 49:1 (ratio)
• 异质活性: DNase (Exonuclease), NICKase (Endonuclease), RNase, protease, none detected
• SMILES字符串: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

说明

应用

即复溶干粉混合物精确预混合，得到 40% (w/v) 贮备液，用于蛋白质和核酸电泳。该浓度基于丙烯酰胺和双丙烯酰胺的总重量。

Acrylamide/Bis-acrylamide has been used in the electrophoresis of SSCP (single-strand conformation)/heteroduplex.

生化/生理作用

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

特点和优势

无需称量有毒丙烯酰胺和双丙烯酰胺。

重悬

向容器中缓慢加入规定量的蒸馏水复溶，并溶解内容物。

To make up 100 ml of A0924, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

安全信息

• 象形图: GHS06,GHS08
• 警示用语：: Danger
• 危险声明: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• 预防措施声明: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• 危险分类: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• 靶器官: Peripheral nervous system
• 储存分类代码: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges