The 96992-3000TESTS vial will include 30 milliliters of reagent.
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應用
Cell Counting Kit-8 (CCK-8)细胞计数试剂盒采用WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)实现快速检测，其原理是WST-8能在电子载体1-甲氧基PMS的作用下被还原成水溶性甲臜产物。CCK-8溶液可直接加入到细胞中，不需要与其它组分预混。WST-8被细胞内脱氢酶生物还原后生成的橙色甲臜染料能够溶解在组织培养基中，生成的甲臜量与活细胞数量成正比。由于CCK-8溶液非常稳定并且细胞毒性很小，所以可以进行长时间孵育，例如孵育24-48小时。
Cell Counting Kit-8可以对细胞增殖和细胞毒性实验中活细胞数目进行敏感的比色法测定。检测灵敏度高于任何其它四唑盐，如 MTT、XTT或MTS。
Cell Counting Kit-8可以对细胞增殖和细胞毒性实验中活细胞数目进行敏感的比色法测定。检测灵敏度高于任何其它四唑盐，如 MTT、XTT或MTS。
訊號詞
Warning
危險分類
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
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商品
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
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What is the solution volume provided in the "3000 tests"?
1 answer-
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100 Test means 100 wells, so only one 96-well plate?
1 answer-
The reagents provided in kit 96992 are sufficient for 100 tests. A 96 well microplate is not included with the kit. The customer must provide the 96 well plate. Although a solid 96 well plate can be used, a 96 well microplate with removable strips will allow for the most efficient use of the kit.
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How much volume (in milliliters) is present in each vial of the CCK-8 solution?
1 answer-
The volume supplied for Product No. 96992, Cell Counting Kit, is as follows:
•100 tests: 1 ml x 1 vial
•500 tests: 5 ml x 1 vial
•3000 tests: 5 ml x 6 vialsHelpful?
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Is this kit suitable for use with HEK293 or HEK293f cells?
1 answer-
Yes, the 96992 kit can be utilized to stain both HEK293 and HEK293B cells. The Cell Count kit is not cell type-specific and is expected to function effectively for counting viable mammalian cells. In this kit, the WST-8 dye, which is a formazan stain is specific to viable cells and is not present in nonviable cells.
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What are the volumes of reagents included in the 96992-100test-F kit?
1 answer-
Supplier information specifies that the kit contains 6 bottles, each containing 5 ml of solution. Based on the product datasheet, each well of a well-plate requires 10 microliters of solution, indicating that for 3000 tests, the kit should contain 30 ml of solution. Using these calculations, for the 100 test kit, each bottle would contain approximately 167 microliters of solution (5 ml divided by 30 = 167 microliters).
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Hi, what is the solution volume provided in the "500 tests" SKU? Thanks in advance, Aviv.
1 answer-
The 500 test volume is 5 mL. See the links below to the Product datasheet and the Product Troubleshooting Guide for additional information.
Product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdfTroubleshooting Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdfHelpful?
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