跳转至内容
Merck
所有图片(1)

Key Documents

View All Documentation

CGD1

Sigma-Aldrich

细胞生长测定试剂盒，基于MTT法

别名:

Mitochondrial activity assay

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

包裝

pkg of 1 kit

技術

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

應用

cell analysis
detection

檢測方法

colorimetric

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

细胞生长测定试剂盒（基于MTT）设计用于以分光光度法测量细胞生长，作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法，可通过线粒体脱氢酶活性测量活细胞活性。

應用

细胞生长测定试剂盒（基于MTT）已用于：
  • 测定成骨前细胞的细胞活力
  • 正常人前列腺间质和上皮细胞的MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯溴化四唑)检测
  • 测定原代宫颈上皮细胞的细胞活力

包裝

每个试剂盒包括5瓶试剂，每瓶含1 mL 5 mg/ml MTT的RPMI溶液（无酚红）和50 mL MTT溶剂（0.1N盐酸的无水异丙醇溶液）。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加，因此每个试剂盒的测试次数取决于培养容量和/或体积大小。

相關產品

产品编号
说明
价格

訊號詞

Danger

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

53.6 °F - closed cup

閃點（°C）

12 °C - closed cup

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Ahran Pae et al.
The journal of advanced prosthodontics, 6(2), 96-102 (2014-05-21)
This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate
Qiuyu Lin et al.
Journal of cellular and molecular medicine, 22(10), 4640-4652 (2018-07-25)
This study is aimed to investigate the methylation level of candidate genes and its impact on thyroid carcinoma (THCA) development. Infinium Human Methylation 450 BeadChip Arrays by Illumina (Illumina HM450K) was the most popular CpG microarray platform widely used in
Mengxiong Wang et al.
Oncogene, 38(22), 4264-4282 (2019-02-06)
While HER2 and EGFR are overexpressed in breast cancers and multiple other types of tumors, the use of EGFR and/or HER2 inhibitors have failed to cure many cancer patients, largely because cancers acquire resistance to HER2/EGFR-specific drugs. Cancers that overexpress
Mariarita Perri et al.
Biochimica et biophysica acta, 1800(9), 993-1001 (2010-07-06)
Vitamin A is suggested to be protective against oxidative stress. However, different authors observed pro-oxidant effects of retinoids both in experimental works and clinical trials. These discordances are the bases for the investigation of the proliferative and anti-proliferative properties of
Shang-Long Liu et al.
Oncology letters, 17(2), 2057-2062 (2019-01-25)
The biological features of pancreatic cancer and the associated hypoxic environment around the cancer cells often lead to resistance to radiotherapy and chemotherapy. The present study was performed in order to explore the effect pancreatic stellate cells (PSCs) have on
查看所有相关文献

商品

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门