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Merck

88417

Sigma-Aldrich

MTT 甲臜

MTT reduction end product, powder

别名:

1-(4,5-二甲基吡啶-2-基)-3,5-二苯基甲酰胺, 噻唑基蓝甲臜

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About This Item

经验公式(希尔记法):
C18H17N5S
CAS号:
分子量:
335.43
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

MTT 甲臜, MTT reduction end product

品質等級

形狀

powder

溶解度

chloroform: 10 mg/mL, clear, dark violet

SMILES 字串

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI 密鑰

GIFKVQOPAJDJLO-SZVLODPRSA-N

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應用

MTT甲臜用于细胞活率检测。

生化/生理作用

MTT法是一种比色反应分析方法,常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化,仅在活细胞中发生。因此,蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。

其他說明

评审:四唑盐和甲瓒

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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