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Merck

38361

Sigma-Aldrich

Abberior® STAR 440SXP, maleimide

for long Stokes STED and 2-color STED application

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About This Item

分類程式碼代碼:
12352111
NACRES:
NA.32

品質等級

化驗

≥80.0% (degree of coupling)

分子量

Mw 622.6 g/mol

溶解度

DMF: 1 mg/mL, clear

螢光

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

應用

Abberior® STAR 440SXP has been conjugated with secondary anti-mouse antibody for dual colour STED (stimulated emission depletion) microscopy. Again, it has been labelled with secondary antibody for STED microscopy in primary hippocampal cells prepared from E18 Sprague Dawley embryos.

適合性

Designed and tested for fluorescent super-resolution microscopy

法律資訊

abberior is a registered trademark of Abberior GmbH

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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BMC neuroscience, 15, 45-45 (2014-03-29)
Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution. Here we have analyzed the spatial relationship between Norbin
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Cell host & microbe, 14(4), 468-480 (2013-10-22)
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to
Volker Westphal et al.
Physical review letters, 94(14), 143903-143903 (2005-05-21)
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly

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