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Merck
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主要文件

H2899

Supelco

HPLC 蛋白标准混标

analytical standard

别名:

蛋白校准混标

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About This Item

分類程式碼代碼:
85151701
NACRES:
NA.24
价格与库存信息目前不能提供

等級

analytical standard

品質等級

形狀

solid

分析物化學類別

amino acids, peptides, proteins

技術

HPLC: suitable

應用

food and beverages

形式

multi-component solution

儲存溫度

−20°C

應用

使用高效液相色谱法 (HPLC) 分离细菌细胞提取物样品中的蛋白质时,使用 HPLC 蛋白标准品混合物作为标准品。[1]

包裝

样品瓶,含核糖核酸酶 A、细胞色素 c、转铁蛋白、脱辅基肌红蛋白各约 1mg。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Distribution, diversity, and activities of sulfur dioxygenases in heterotrophic bacteria
Liu H, et al.
Applied and Environmental Microbiology, 80, 1799-1806 (2014)
Honglei Liu et al.
Applied and environmental microbiology, 80(5), 1799-1806 (2014-01-07)
Sulfur oxidation by chemolithotrophic bacteria is well known; however, sulfur oxidation by heterotrophic bacteria is often ignored. Sulfur dioxygenases (SDOs) (EC 1.13.11.18) were originally found in the cell extracts of some chemolithotrophic bacteria as glutathione (GSH)-dependent sulfur dioxygenases. GSH spontaneously

Questions

1–2 of 2 Questions  
  1. Could you provide an order of retention times of when each of these components elute off a protein C4 column (H2899 protein mixture)?  Thank you.

    1 answer
    1. The exact method is considered proprietary. However, for a C4 column, it should elute ribonuclease A first, followed by cytochrome C, holo-transferrin, and apomyoglobin.

      Helpful?

  2. What is each componant's actual molecular weight (ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin)?

    1 answer
    1. The molecular weight of each component is not reported. See below for the expected values:
      Ribonuclease A: approx. 13.7 kDa
      Cytochrome C: approx. 12.3 kDa
      holo-Transferrin: 72.1 - 78.5 kDa
      Myoglobin: approx. 17.6 kDa

      Helpful?

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