Direct Red 80 is not performance tested for use in a Picro Sirius Red staining method. Tissues fixed or stained with Picric Acid may require long rinse or wash steps to remove excess picric acid, and using slightly acidified water has been seen in protocols to remove the excess picric acid from tissue sections.
价格与库存信息目前不能提供
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表单
powder
质量水平
组成
Dye content, 25%
技术
titration: suitable
颜色
dark brown
λmax
528 nm
ε (消光系数)
≥12000 at 277-283 nm in H2O
≥15000 at 223-235 nm in H2O
≥32000 at 524-530 nm in H2O
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O
InChI
1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;
InChI key
YIQKLZYTHXTDDT-IJPLDVMMSA-H
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一般描述
Direct Red 80（C.I.35780）是一种极亲水、非常大的四偶氮六磺酸阴离子染料，具有大的平面芳香体系。它也被称为天狼星红 F3B、氯胺坚牢红 7BLN 或 5BRL、杜拉唑亮红 B、滂胺坚牢红 7BNL 和天狼星红。
应用
直接红 80 已被用作制备饱和苦味酸的成分，用于免疫组织化学检测和定量肝组织中的胶原蛋白。其他应用程序包括-
- 淀粉样蛋白的检测
- 使用图像分析方法定量胶原的天狼星红瑞德-范吉森法
- 在明矾苏木精中取代曙红的复染应用
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Using Direct Red 80 with Picric acid P6744 for Picro Sirius Red staining, and the staining resultss are coming out very yellow. Any advice on this issue?
1 answer-
Helpful?
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Is Direct Red 80 comparable to Dextran Red for back-filling injections in live zebrafish spinal cord?
1 answer-
Direct Red 80 is more commonly referred to as Sirius Red and is a stain for collagen. It is a vital stain used for various types of biological staining. Dextran Red is a fluorescent dye for which you would require a fluorescent microscope to view the staining. The different molecular weights of dextran are used to determine whether the dye crosses certain barriers.
Helpful?
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