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等級
ACS reagent
品質等級
形狀
powder
技術
titration: suitable
visual transition interval
3.0-4.6, yellow to blue
mp
273 °C (lit.)
溶解度
ethanol: 0.1%
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI 密鑰
UDSAIICHUKSCKT-UHFFFAOYSA-N
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一般說明
溴酚蓝在酸性水溶液中以磺化羟基醌的形式存在。又名四溴酚磺酞。溴酚蓝是一种pH指示剂,并且是一种强蓝色染料。 它带有轻微的负电荷并且与DNA同向迁移,方便用户对分子在凝胶中的移动进程进行监测。不同的凝胶组成具有不同的迁移速率。
應用
溴酚蓝可用作DNA、RNA(琼脂糖)和蛋白(聚丙烯酰胺)凝胶电泳中的示踪染料。它被用于为蛋白质印迹分析制备蛋白质样品。溴酚蓝在琼脂糖凝胶中以与300-500 bp DNA大致相同的速率迁移,在蛋白聚丙烯酰胺凝胶中位于缓冲前沿。 还用作血脑屏障的重要检测染料。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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