This product is a 1 mg/mL solution. The enzymatic activity of each component is as follows:
Dispase Activity: 1.0 units per milligram
Collagenase Activity: ≥12,500 units per vial
Caseinase Activity: ≥2000 caseinase units/mg
Clostripain Activity: ≤5.0 clostripain units/mg
Tryptic Activity: ≤0.5 tryptic units/mg
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质量水平
表单
liquid
制造商/商品名称
PluriSTEM®
浓度
1 mg/mL in sterile DMEM/F-12
技术
cell culture | mammalian: suitable
cell culture | stem cell: suitable
运输
dry ice
一般描述
已验证PluriSTEM 分散酶-II可与PluriSTEM人ES/iPS培养基(SCM130)一起用于人胚胎和诱导多能干细胞的培养和传代。
分散酶-II在多粘芽孢杆菌中产生。
应用
1.用1:20 Matrigel涂层(每孔1.5 ml)涂覆6孔板。 在2-8ºC孵育过夜或在室温涂覆至少30分钟至1小时后再使用。
2.去除基质胶涂层。 每孔添加2 mL完全培养基。
3.去除培养物中的分化区域。
4.吸出培养基。
5.用2 mL PBS洗涤一次。
6.每孔加入1.5 mL 分散酶II。 37ºC孵育7分钟。
7.用2 mL不含Mg和Ca的PBS洗涤2次。
8.向每个孔中加入2 mL完全培养基,并使用细胞刮刀分离菌落。
9.将刮下的细胞收集在15 ml锥形管中。
10800 rpm离心5分钟
11.重悬在适当体积的完全培养基中。 典型的稀释比为1:5 – 1:6,其取决于细胞密度。 细胞在5-6天内可使用PluriSTEM Human ES/iPS培养基进行传代。
酶&生物化学
干细胞研究
质量
外形
Dispase-II酶是在多粘芽孢杆菌中产生的。
储存及稳定性
法律信息
免责声明
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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商品
Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.
Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.
Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.
Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.
实验方案
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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What is the activity of this enzime?
1 answer-
Helpful?
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What is the activity of the dispase product SCM133, and is there a specific range it should fall within in units/mL?
1 answer-
The activity of the material is 1.0 unit per milligram (u/mg).
Helpful?
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Please let me know U/ml of this (https://www.sigmaaldrich.com/CZ/en/product/mm/scm133) solution. Thank you
1 answer-
This product is a 1 mg/mL solution of Dispase II. The enzyme activity is lot specific, however the activity will be ?0.8 U/mg. This activity is established using casein as a substrate at 37°C and pH 7.5. Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/727/543/scm133.pdfHelpful?
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