The thickness of the polycarbonate and PET membranes range from 7-20 um. The standing inserts (PIHP01250) have small "feet" that contact the bottom of the wells. The hanging inserts (PTHT12H48) are suspended by 'wings' that rest on the sides of the wells. Please see the image below:
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材料
polystyrene housing
translucent PET membrane
品質等級
無菌
γ-irradiated
sterile
特點
100x106 pores/cm2
包裝
pack of 48 ea (individually blister packaged)
製造商／商標名
Millicell®
參數
37 °C max. temp.
技術
cell attachment: suitable
cell differentiation: suitable
permeability assay: suitable
高度
15.5 mm
直徑
12 mm
insert maximum volume
0.8 mL
小室有效容積
0.4 mL
尺寸
12 wells
表面積
1.1 cm2
顏色
translucent membrane
孔徑
0.4 μm
結合類型
Tissue Culture (TC)-treated surface
相关类别
一般說明
Millicell®悬挂式细胞培养小室配备聚对苯二甲酸乙二酯（PET）膜，特别适合各种细胞类型的生长和分化。这种独立灭菌和包装的培养小室具有0.4 μm膜孔径，适合大多数12孔塑料组织培养板。
在塑料组织培养板中，细胞只能从顶侧接触培养基，但是在Millicell®组织培养小室中，细胞可以从顶侧和底侧接触培养基。结果使细胞生长、结构和功能更能模拟体内的情形。
培养小室的法兰设计使其悬浮在培养板孔内部，并且可以接触细胞单层的两侧。 三个突起臂（protruding arm）之间的空间有助于培养小室外侧和培养板孔之间的移液。
在塑料组织培养板中，细胞只能从顶侧接触培养基，但是在Millicell®组织培养小室中，细胞可以从顶侧和底侧接触培养基。结果使细胞生长、结构和功能更能模拟体内的情形。
培养小室的法兰设计使其悬浮在培养板孔内部，并且可以接触细胞单层的两侧。 三个突起臂（protruding arm）之间的空间有助于培养小室外侧和培养板孔之间的移液。
應用
- 细胞增殖
- 细胞表面受体
- 细胞培养
- 上皮细胞生长
- 饲养层
- 荧光检测/免疫组织化学
- 体外 毒理学
- 微生物附着
- 极化蛋白分泌
- 极化摄取
- 运输/渗透性
法律資訊
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
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实验方案
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Regarding the PIHP01250 & PTHT12H48 membrane insert products: what is the thickness of the membrane? Is the bottom totally flat, i.e. if I placed it down on a flat surface, would the entire area of the membrane be in direct contact with the surface?
1 answer-
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