推荐产品
生物源
mouse
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
162 33 D3, monoclonal
形狀
liquid
不包含
preservative
物種活性（以同源性預測）
all
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG1
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
免疫亲和纯化的小鼠单克隆抗体。识别5-甲基胞嘧啶。
经验证，该抗5-甲基胞嘧啶小鼠mAb（162 33 D3）可用于ELISA、FC、冷冻切片、IF、石蜡切片、放射免疫分析、DNA-蛋白质印迹法检测5-甲基胞嘧啶。
识别NIH3T3细胞中甲基化DNA或RNA中的5-甲基胞嘧啶。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
特异性
•亲和纯化的小鼠单克隆抗体 - 可检测多种物种的甲基化DNA。几种应用
• 流式细胞术
•冷冻切片
• 免疫印迹
•免疫荧光
•石蜡切片
可靠
•产生可重复的结果。
•克隆162 33 D3已在50多个出版物中使用。
免疫原
5-甲基胞嘧啶结合卵清蛋白
應用
ELISA（0.1 g/mL）
流式细胞术（1 g/mL）
冷冻切片（5-10 g/mL）
免疫印迹法（5 g/mL）
免疫荧光（1 g/mL）
石蜡切片（5-10 g/mL）
放射免疫分析（0.1 g/mL）
DNA-蛋白质印迹法（5 g/mL）
包裝
批次特定浓度请参见小瓶标签。
警告
毒性：标准处理（A）
外觀
在PBS（pH 7.4）中。
重構
初次解冻后，等分并冷冻（-20°C）。
分析報告
阳性对照
NIH3T3细胞
NIH3T3细胞
其他說明
Ehrlich, M. 2002.Oncogene21, 5400.(Review)
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
Widschwendter, M. and Jones, P.A.2002.Oncogene21, 5462.(Review)
Barton, S.C., et al. 2001.Hum.Mol.Genet.10, 2983.
Piyathilake, C.J., et al. 2001.Hum.Pathol.32, 856.
Taddei, A., et al. 2001.Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000.Biotech.Histochem.75, 251.
De Capoa, A., et al. 1999.FASEB J.13, 89.
克隆162 33 D3可用于定量和定性检测各种样品和应用中的甲基化DNA或RNA。它已在40多个出版物中使用。应滴定抗体，以在单个系统中获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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