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Merck

B6916

Supelco

Bradford试剂

for 0.1-1.4 mg/ml protein

别名:

考马斯染料结合蛋白检测,蛋白染料试剂

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About This Item

分類程式碼代碼:
12161500
NACRES:
NA.32

品質等級

形狀

solution

儲存溫度

2-8°C

相关类别

一般說明

Bradford检测是把考马斯亮蓝G-250加入至蛋白溶液中。考马斯蓝染料可与碱性和芳香族氨基酸结合,从而在蛋白质测定过程中引起吸光值的迁移。

應用

Bradford试剂已用于测定总蛋白的浓度。

特點和優勢

  • 该试剂为即用型。 无需混合或稀释。
  • 显色很快速。 仅需五分钟的孵育便能将样本在595 nm处进行读值。
  • 带有硫醇的还原性糖和还原性底物并不会干扰该试剂。
  • 试剂适用于微量(1-10 μg/ml)及标准(50-1400 μg/ml)的检测。
  • 可用于微孔板检测。
  • 低成本检测。

法律資訊

應用

产品编号
说明
价格

相關產品

产品编号
说明
价格

象形圖

Health hazardCorrosion

訊號詞

Warning

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

標靶器官

Eyes,Central nervous system

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

实验方案

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

相关内容

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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