跳转至内容
Merck
所有图片(6)

文件

MAB4360

Sigma-Aldrich

抗-TRA-1-60抗体，克隆TRA-1-60

clone TRA-1-60, Chemicon®, from mouse

别名:

TRA-1-60 Marker

登录查看公司和协议定价
所有图片(6)

About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

100

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

TRA-1-60, monoclonal

物種活性

human

製造商／商標名

Chemicon®

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

輸入

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

同型

IgM

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... PODXL(5420)

相关类别

一般說明

人胚胎性癌(EC)细胞是畸胎癌的干细胞，它们是生殖细胞肿瘤(GCT)的关键组成部分。它们表达几种在分化时被下调的高分子量糖蛋白抗原。可以在GCT患者的血清中检测到由单克隆抗体TRA-1-60定义的这些抗原之一，并且可以为已建立的血清标志物人绒毛膜促性腺激素和α-胎蛋白提供有用的补充，特别是在那些血清人绒毛膜促性腺激素或α-胎蛋白没有升高的患者中。

特異性

该抗体与在人四癌干细胞(EC)、人胚胎生殖细胞(EG)和人胚胎干细胞(ES)表面表达的TRA-1-60抗原反应。 鼠EC、EG或ES细胞未见免疫反应性。 TRA-1-60和TRA-1-81单克隆抗体（MAB4381）均可识别与细胞周围基质蛋白聚糖相关的抗原。 TRA-1-60与唾液酸酶敏感表位反应，而TRA-1-81与同一分子的未知表位反应。

免疫原

人胚胎性癌细胞系2102Ep

應用

免疫荧光：一个先前的批次被用于IF。

蛋白质印迹法： 该抗体的一个先前批次被用于WB。

免疫沉淀：该抗体的一个先前批次被用于IP。

免疫组织化学： 该抗体的一个先前批次被用于IH。

流式细胞术: 建议起始范围为10-20 µg/mL。

最佳工作稀释度必须由最终用户确定。
研究子类别
多能性&早期分化
研究类别
干细胞研究
经验证，该抗TRA-1-60抗体（克隆TRA-1-60）可用于WB、FC、IF、IP、IC检测TRA-1-60。

標靶描述

235/410 kDa

外觀

形式：纯化
纯化的小鼠单克隆IgM液体，溶于0.05 M磷酸钾、0.3 M NaCl pH 8.0和0.05%叠氮化钠中。

儲存和穩定性

自接收之日起1年内保持稳定。

分析報告

对照
NTERA-2 cl.D1全细胞裂解物（源自畸胎癌的多能干细胞，被认为是人类胚胎干细胞的恶性对应物）

其他說明

浓度：关于批次特定浓度请参见检验报告。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

未找到合适的产品？  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Sriram Bandi et al.
Journal of cell science, 125(Pt 5), 1274-1283 (2012-02-22)
Understanding the identity of lineage-specific cells arising during manipulations of stem cells is necessary for developing their potential applications. For instance, replacement of crucial functions in organ failure by transplantation of suitable stem-cell-derived cells will be applicable to numerous disorders
Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis.
Muller, T; Eildermann, K; Dhir, R; Schlatt, S; Behr, R
Human Reproduction null
Mahdieh Jadaliha et al.
PloS one, 7(6), e38532-e38532 (2012-06-23)
Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through
Tomoko Andoh-Noda et al.
Molecular brain, 8, 31-31 (2015-05-28)
Rett syndrome (RTT) is one of the most prevalent neurodevelopmental disorders in females, caused by de novo mutations in the X-linked methyl CpG-binding protein 2 gene, MECP2. Although abnormal regulation of neuronal genes due to mutant MeCP2 is thought to
Livia Eiselleova et al.
The International journal of developmental biology, 52(4), 353-363 (2008-04-17)
Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, feeder cells differ in their capacity to support the growth of undifferentiated
查看所有相关文献

商品

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门