MAB19101

Anti-Tenascin Antibody, clone DB7

clone DB7, Chemicon^®, from mouse

• 别名: Tenascin-C
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: DB7, monoclonal
• 种属反应性: human, rabbit, guinea pig
• 请勿与下列物质发生反应: rat, mouse
• 制造商/商品名称: Chemicon^®
• 技术: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG2a
• UniProt登记号: P24821
• 运输: wet ice
• 靶向翻译后修饰: unmodified
• 基因信息: human ... TNC(3371)

说明

特异性

The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein

免疫原

Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.

应用

Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).

Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.

Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.

Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.

Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)

Final dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

联系

Replaces: MAB1902

外形

Format: Purified

The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.

储存及稳定性

Maintain refrigerated at 2-8°C

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable