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EZIL6

Sigma-Aldrich

Human IL-6 ELISA Kit

detect IL-6 in 50 μL serum, plasma or cell culture supernatent

别名:

B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IL6, Interferon beta-2, Interleukin-6

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.77

product name

Human IL-6 Conferma® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples

品質等級

物種活性

human

參數

50 μL sample volume (4hr assay)

assay range

linearity: 105-116%
(serum)

linearity: 88-105%
(plasma)

inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%

技術

ELISA: suitable

輸入

sample type serum
sample type plasma (K2 EDTA)
sample type cell culture supernatant

UniProt登錄號

應用

research use

檢測方法

colorimetric (450nm/590nm)

基因資訊

human ... IL6(3569)

一般說明

This Human Interleukin-6 (IL-6) Conferma® ELISA kit is used for the quantification of Human IL-6 in serum, plasma, or culture media samples. Human IL-6 is a soluble protein cytokine most commonly associated with the acute inflammatory response to infection or tissue damage.

This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs

Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.

UniProt Summary:
  • Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
  • Size: 212 amino acids; 23,718 Da
  • Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
  • Subcellular location: Extracellular region or secreted
  • Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Similarity: Belongs to the IL-6 superfamily.

應用

One kit is sufficient to measure 37 samples in duplicate

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

分析報告

3 hour 45 minute assay, room temperature, 50 μL sample size (serum, plasma, or tissue culture medium)

法律資訊

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

For research use only. Not for use in diagnostic procedures.

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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商品

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

实验方案

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

该文章提供了4种常用的ELISA实验方法:夹心法ELISA实验方法、磷酸化检测过程、EIA检测过程以及基于细胞的检测过程

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