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Merck
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69053

Sigma-Aldrich

BLR(DE3)感受态细胞- Novagen

Escherichia coli, rod shaped

别名:

Competent cells for cloning

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.81

product name

BLR(DE3)感受态细胞- Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.

生物源

Escherichia coli

品質等級

製造商/商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

transformation efficiency: >2×106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

基因型:F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)





本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明(EUD)表格。
BLR是BL21的recA-衍生物,可提高质粒单体产量,并有助于稳定含有重复序列,或其产物可能导致DE3原噬菌体丢失的靶质粒(1,2)。

DE3表明宿主是λDE3的溶酶原,因此携带一个由lacUV5启动子控制的T7 RNA聚合酶基因的染色体拷贝。这些菌株适合于通过IPTG诱导从pET载体中克隆的靶基因生产蛋白质。
BLR是BL21的recA衍生物,可提高质粒单体产量,并有助于稳定含有重复序列的靶质粒。

成分

0.4 ml1 ml成分

•2 × 0.2 ml5 × 0.2 mlBLR(DE3)感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng测试质粒

警告

毒性:多个毒性值,请参阅MSDS(O)

其他說明

1.A. Roca (U. of Wisconsin), personal
communication.

2.Studier, F. W. (1991) J. Mol.Biol. 219,
37-44.

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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Jugal P Dhandhukia et al.
Bioconjugate chemistry, 28(11), 2715-2728 (2017-09-25)
Recombinant protein-polymer scaffolds such as elastin-like polypeptides (ELPs) offer drug-delivery opportunities including biocompatibility, monodispersity, and multifunctionality. We recently reported that the fusion of FK-506 binding protein 12 (FKBP) to an ELP nanoparticle (FSI) increases rapamycin (Rapa) solubility, suppresses tumor growth
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Molecular cell, 72(3), 541-552 (2018-11-06)
Numerous classes of riboswitches have been found to regulate bacterial gene expression in response to physiological cues, offering new paths to antibacterial drugs. As common studies of isolated riboswitches lack the functional context of the transcription machinery, we here combine
U Loi Lao et al.
Nature protocols, 2(5), 1263-1268 (2007-06-05)
This protocol presents a new method to purify plasmid DNA using temperature-triggered precipitation. The principle is based on the specific DNA-binding affinity of a bacterial metalloregulatory (MerR) protein to its cognate DNA sequence and the temperature responsiveness of elastin-like protein
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During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function
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Theranostics, 7(16), 3856-3872 (2017-11-08)
Recombinant Elastin-Like Polypeptides (ELPs) serve as attractive scaffolds for nanoformulations because they can be charge-neutral, water soluble, high molecular weight, monodisperse, biodegradable, and decorated with functional proteins. We recently reported that fusion of the FK-506 binding protein 12 (FKBP) to

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