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用途
sufficient for 200 precipitation(s)
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
運輸包裝
ambient
儲存溫度
10-30°C
一般說明
一种方便且高度可重复的试剂盒,用于沉淀和清除蛋白质。沉淀蛋白的产率高于传统方法,并且可改善沉淀蛋白的溶解度。包含足够沉淀200个≤200 µl样品的组成成分。需要150 ml乙醇(未提供)。试剂盒可提供极低电导率的蛋白质溶液。
成分
沉淀剂1、沉淀剂2、沉淀剂3、沉淀剂4、洗涤液和用户实验方案。
警告
毒性:多个毒性值,参见MSDS(O)
原則
ProteoExtract 蛋白质沉淀试剂盒专门用于浓缩和清除水性样品中的蛋白质。
準備報告
• 样品:该方法可从多种样品(含50 µg/ml至10 mg/ml蛋白质)中对蛋白进行沉淀。该方法可耐受高达8 M的离液剂(例如尿素)、高达4%的去污剂、和盐高达2 M的盐溶液。
注意:首次使用该试剂盒之前,必须提前准备沉淀剂和洗涤液并将其冷却至-20 °C。•沉淀剂: 1.向沉淀剂1中添加1.7 ml沉淀剂2、1.7 ml沉淀剂3和1.7 ml沉淀剂4;混匀。2.沉淀剂1、2、3和4的混合物称为沉淀剂。一瓶沉淀剂(34.1 ml)足够用于沉淀40个200 µl的样品。3.重新溶解后,将沉淀剂1的瓶上贴上标签,以便于识别。•为了易于使用,请将沉淀剂存放在-20 °C;重溶后的沉淀剂可稳定长达2个月。•洗涤液:1在洗涤液瓶中加入150 ml优质乙醇(未提供)(也可以使用变性乙醇)2.混匀并储存在-20 °C。重溶的洗涤液可稳定使用一年。注意:每200 µl样品需要800 µl沉淀剂和1 ml洗涤液。
儲存和穩定性
收到货后,试剂盒的所有组分均可在室温下保存(20 °C)。为了方便使用,配制好的沉淀剂和洗涤液可以在-20 °C下保存(请参阅试剂制备部分)。
其他說明
由于该运输中有害物质的性质,您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系,以获取有关这些费用的更多信息。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
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