444256

MMP Substrate III, Fluorogenic

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

• 别名: MMP Substrate III, Fluorogenic, DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
• 经验公式（希尔记法）: C₆₃H₈₈N₁₆O₁₄S
• 分子量: 1325.54
• 分類程式碼代碼: 12352200
• NACRES: NA.77

属性

• 品質等級: 100
• 化驗: ≥97% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: red
• 溶解度: DMSO: soluble; Tris-buffered saline, pH 7.6: soluble
• 螢光: λ_ex ~340 nm; λ_em ~485 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, 21,000 M^-1s^-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, and 21,000 M^-1s^-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.

生化／生理作用

Cell permeable: no

Primary Target
Detection of MMP activity

Product does not compete with ATP.

Reversible: no

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

序列

DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

外觀

Supplied as a trifluoroacetate salt.

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他說明

Beekman, B., et al. 1996. FEBS Lett390, 221.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable