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Key Documents

400064

Sigma-Aldrich

Anti-Glucose Transporter-4 Rabbit pAb

liquid, Calbiochem®

别名:

Anti-Glut-4

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

rat, human, mouse

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... SLC2A4(6517)

一般說明

Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
Recognizes the ~40-43 kDa GLUT-4 protein.

免疫原

Mouse
a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH

應用

ELISA (0.5-1 µg/ml)

Frozen Sections (2-20 µg/ml)

Immunoblotting (1-10 µg/ml; see comments)

Immunoprecipitation (1-10 µg/100 µg tissue membrane)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In PBS, 0.1% BSA, pH 7.5.

重構

Following initial thaw, aliquot and freeze (-20°C).

其他說明

Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Malcolm Grohmann et al.
The Journal of physiology, 568(Pt 1), 229-242 (2005-08-06)
We have developed a primary skeletal muscle cell culture model derived from normal prepubertal children to investigate the effects of insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 (IGFBP-3) and tumour necrosis factor alpha (TNFalpha) on growth, differentiation and
Xue-Han Ning et al.
American journal of physiology. Heart and circulatory physiology, 292(1), H333-H341 (2006-09-05)
Hypoxia-inducible factor 1alpha (HIF-1alpha) transcriptionally activates multiple genes, which regulate metabolic cardioprotective and cross-adaptive mechanisms. Hypoxia and several other stimuli induce the HIF-1alpha signaling cascade, although little data exist regarding the stress threshold for activation in heart. We tested the
Jae-Heung An et al.
Journal of exercise rehabilitation, 12(3), 163-170 (2016-07-16)
The purpose of this study was to investigate the expression of lipogenesis- and lipolysis-related genes and proteins in skeletal muscles after 12 weeks of resistance training. Sprague-Dawley rats (n=12) were randomly divided into control (resting) and resistance training groups. A
Eric B Taylor et al.
The Journal of biological chemistry, 283(15), 9787-9796 (2008-02-16)
The Akt substrate of 160 kDa (AS160) is phosphorylated on Akt substrate (PAS) motifs in response to insulin and contraction in skeletal muscle, regulating glucose uptake. Here we discovered a dissociation between AS160 protein expression and apparent AS160 PAS phosphorylation
Young Ju Lee et al.
Molecular medicine reports, 7(5), 1571-1578 (2013-04-03)
Alzheimer's disease (AD) is closely associated with significant defects in glucose metabolism. To investigate whether AD pathology induced by overexpression of human mutant presenilin 2 (PS2) protein induces changes in glucose metabolism, glucose‑related factors were analyzed in the brain of

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