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product name
Chromolith® Si硅胶色谱柱, L × I.D. 100 mm x 10 mm column
材料
PEEK column
品質等級
agency
suitable for USP L3
產品線
Chromolith®
特點
endcapped: no
包裝
1 ea of
參數
150 bar pressure
45 °C max. temp.
技術
HPLC: suitable (Semi Prep HPLC)
LC/MS: suitable
柱長度 × 內徑
10 cm × 10 mm
基質
monolithic silica
基質活性組
Silica bonding phase
孔徑
13 nm mesoporosity
2 μm macroporosity
工作pH值範圍
2-7.5
分離技術
hydrophilic interaction (HILIC)
normal phase
儲存溫度
no temp limit
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一般說明
Chromolith® SemiPrep Si HPLC columns are ideally suited for direct scale-up from analytical to semiprep. This is because they offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles.
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with excellent performance. This makes them the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d.). They have the same bimodal porous silica rod structure as Chromolith® analytical columns with an internal diameter of 4.6 mm. Their macropores are 2 μm in diameter and the mesopores are 13 nm. This combination dramatically reduces separation time while increasing efficiency.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
The recommended Guard-columns are:
Chromolith® SemiPrep Si 10-10 guard cartridges (3 pieces) [1.52035] with the Chromolith® Guard Cartridge Holder 10 mm x 10 mm [1.52037]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
分析報告
Pressure: ≤ 25 bar
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95
Performance (2-nitroanisol)
Theoretical Plates (N/m) (2-nitroanisol): ≥ 80000
Capacity factor (2-nitroanisol): 1.4 - 1.9
Tailing (USP): 0.90 - 1.40 %
Selectivity
separation factor (2-nitroanisol/nitrobenzene): 3.45 - 3.95
法律資訊
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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