推荐产品
生物源
human
品質等級
重組細胞
expressed in Sf21 cells
分子量
α-subunit Mw 49 kDa (heterotetramer; α2β2)
β subunit Mw 53 kDa (heterotetramer; α2β2)
製造商/商標名
Upstate®
技術
activity assay: suitable (kinase)
UniProt登錄號
一般說明
产品来源:在Sf21昆虫细胞中表达。
重组全长人酪蛋白激酶2蛋白,包含&alpyha;-亚基N末端6His标签和β-亚基N末端GST标签。
生化/生理作用
蛋白质靶标:CK2
包裝
2瓶,每瓶5μg
也提供250 μg的规格(2x125μg)-订购250 μg的规格时,请致电咨询价格和供货情况,参考目录号14-197M。
也提供250 μg的规格(2x125μg)-订购250 μg的规格时,请致电咨询价格和供货情况,参考目录号14-197M。
品質
通过酪蛋白激酶2底物肽的磷酸化进行常规评估
外觀
20mM MOPS,pH 7.4,1mM EDTA,0.1%(v/v)Triton X-100,0.3% 2-巯基乙醇,1mM苯甲脒,50%甘油。
使用肝素琼脂糖凝胶™和Mono Q阴离子交换树脂的顺序色谱法
儲存和穩定性
在-20°C下保存1年。
其他說明
比活相关数据,请参阅检验报告以获取该酶的单独批次。
法律資訊
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
In vitro phosphorylation of the polyomavirus major capsid protein VP1 on serine 66 by casein kinase II
The Journal of Biological Chemistry, 270, 26006-26011 (1995)
Casein kinase II beta-subunit inhibits the activity of the catalytic alpha-subunit in the absence of salt.
Biochimica et Biophysica Acta, 1203, 282-289 (1993)
European journal of biochemistry, 229(3), 703-709 (1995-05-01)
Casein kinase II, cyclin-dependent kinases, and glycogen synthase kinase-3 are members of the protein kinase subfamily with a prominent insert in domain X of their catalytic subunit sequence. The function of the insert sequence in casein kinase II was investigated
The Biochemical journal, 352 Pt 1, 145-154 (2000-11-04)
Stress-activated protein kinase 1 (SAPK1), also called c-Jun N-terminal kinase (JNK), becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif
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