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Merck
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Key Documents

1.01167

Sigma-Aldrich

Amido black 10 B (C.I.20470)

for electrophoresis

别名:

Amido black 10 B (C.I.20470), Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black

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About This Item

经验公式(希尔记法):
C22H14N6Na2O9S2
CAS号:
分子量:
616.49
MDL號碼:
分類程式碼代碼:
41105305
酶委員會索引編號:
213-903-1

品質等級

形狀

solid

效力

>5000 mg/kg LD50, oral (Rat)

成份

Dye, ≥80% spectrophotometric assay

pH值

9.0 (25 °C, 10 g/L in H2O)

溶解度

10 g/L

體積密度

670 kg/m3

εmax

750-960 at 617-620 nm in water at 0.01 g/L

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

2-30°C

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,24H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25?,27-21+;;

InChI 密鑰

HKBVRFLHNUEVRO-DWTBGCDMSA-L

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一般說明

Amido black 10 B (C.I. 20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

分析報告

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 750 - 960
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for electrophoresis: passes test
Suitability as redox indicator: passes test

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°C)

Not applicable


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