05-163

Anti-PLCγ-1 Antibody

Upstate^®, from mouse

• 别名: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, Phosphoinositide phospholipase C, Phospholipase C-gamma-1, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phospholipase C gamma 1,
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: B-2-5, monoclonal; B-20-3, monoclonal; B-6-4, monoclonal; D-7-3, monoclonal; E-9-4, monoclonal
• 物種活性: mouse, human, bovine, rat, rabbit
• 製造商／商標名: Upstate^®
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_002660.2
• UniProt登錄號: P19174
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PLCG1(5335)

说明

一般說明

Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

特異性

This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).

免疫原

Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.

應用

Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.

Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.

Research Category
Signaling

Research Sub Category
Lipid Signaling

品質

Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.

標靶描述

135 kDa

外觀

Protein G Chromatography

Format: Purified

Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

儲存和穩定性

Stable for 1 year at -20ºC from date of shipment.

分析報告

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable