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描述
Methacrylated collagen:
Degree of methacrylation ≥ 20%
Product components :
品質等級
無菌
sterile; sterile-filtered
形狀
liquid
雜質
≤10 EU/mL Endotoxin
儲存溫度
2-8°C
應用
PhotoCol™-LAP生物墨水试剂盒以纯化的甲基丙烯酸I型牛胶原蛋白为核心组分,配合其他负载试剂。甲基丙烯酸型胶原蛋白是由端肽完整的牛胶原蛋白产生的,胶原蛋白是通过反应游离胺来修饰的,主要是赖氨酸残基的ε-胺基团以及N端的a-胺基团。胶原蛋白分子中超过20%的赖氨酸残基被甲基丙烯酸化。提供一瓶20 mM醋酸溶液来溶解冻干的甲基丙烯酸胶原蛋白,浓度从3到8 g/ml不等。中和溶液由10X磷酸盐缓冲盐水(PBS)溶液组成,该溶液提供最终混合物中的生理盐和pH值。光引发剂由LAP组成,在1X细胞培养基或PBS中配制,可使打印结构在405 m下进行蓝光光交联。PhotoCol™-LAP提供原生型3D胶原蛋白凝胶,通过改变胶原蛋白浓度和交联,可以定制最终凝胶的刚度。
法律資訊
PhotoCol is a trademark of Advanced BioMatrix, Inc.
儲存類別代碼
10 - Combustible liquids
A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues.
Advanced Biosystems, 1700237-1700237 (2018)
Langmuir : the ACS journal of surfaces and colloids, 30(37), 11204-11211 (2014-09-11)
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Type-I collagen is an attractive scaffold material for tissue engineering due to its ability to self-assemble into a fibrillar hydrogel, its innate support of tissue cells through bioactive adhesion sites, and its biodegradability. However, a lack of control of material
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