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Merck

916293

Sigma-Aldrich

PhotoCol-LAP

methacrylated collagen bioink kit, with LAP

别名:

3D生物打印, 生物墨水, 胶原蛋白

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About This Item

分類程式碼代碼:
12352201
NACRES:
NA.23

描述

Methacrylated collagen:
Degree of methacrylation ≥ 20%

Product components :

  • Methacrylated collagen (100 mg)
  • 20 mM acetic acid (50 mL)
  • Neutralization solution (10 mL)
  • LAP photoinitiator (100 mg)

品質等級

無菌

sterile; sterile-filtered

形狀

liquid

雜質

≤10 EU/mL Endotoxin

儲存溫度

2-8°C

應用

PhotoCol-LAP生物墨水试剂盒以纯化的甲基丙烯酸I型牛胶原蛋白为核心组分,配合其他负载试剂。甲基丙烯酸型胶原蛋白是由端肽完整的牛胶原蛋白产生的,胶原蛋白是通过反应游离胺来修饰的,主要是赖氨酸残基的ε-胺基团以及N端的a-胺基团。胶原蛋白分子中超过20%的赖氨酸残基被甲基丙烯酸化。提供一瓶20 mM醋酸溶液来溶解冻干的甲基丙烯酸胶原蛋白,浓度从3到8 g/ml不等。中和溶液由10X磷酸盐缓冲盐水(PBS)溶液组成,该溶液提供最终混合物中的生理盐和pH值。光引发剂由LAP组成,在1X细胞培养基或PBS中配制,可使打印结构在405 m下进行蓝光光交联。PhotoCol-LAP提供原生型3D胶原蛋白凝胶,通过改变胶原蛋白浓度和交联,可以定制最终凝胶的刚度。

法律資訊

PhotoCol is a trademark of Advanced BioMatrix, Inc.

儲存類別代碼

10 - Combustible liquids


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Type-I collagen self-assembles into a fibrillar gel at physiological temperature and pH to provide a cell-adhesive, supportive, structural network. As such, it is an attractive, popular scaffold for in vitro evaluations of cellular behavior and for tissue engineering applications. In
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