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應用
cell analysis
一般說明
3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321: CC321-1, CC321-2) is based upon purified type I bovine collagen methacrylate (ColMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to type I bovine collagen methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and other reagents for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen that has been modified by reacting with the protein′s free amines, primarily the ɛ-amines groups of the lysine residues as well as the α-amines groups on the N-termini. >20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content that was isolated from a closed herd and purified using controlled manufacturing processes.PhotoCol is a registered trademark of Advanced BioMatrix, Inc.
應用
Photocrosslinking is initiated using a 405 nm blue light source.
包裝
1 Kit
成分
CC321-1 (Store at Room Temp): Neutralization Solution, 1 X 10 mL (CS226411).
Note: The complete PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321) consists of CC321-1 and CC321-2 sold seperately.
Note: The complete PhotoCol-LAP, Methacrylated Collagen Hydrogel Kit (CC321) consists of CC321-1 and CC321-2 sold seperately.
品質
Sterility (USP modified): No GrowthEndotoxin LAL: ≤ 10.0 EU/mlGel Formation: ≤ 40 minKinetic Gel Test: ≤ 40 minPurity (SDS Page Silver Stain): ≥ 99%Degree of Methacrylation: ≥ 20%pH: 3.0-4.0Osmolality: ≤ 40 mOsm/Kg H20Young′s Modulus: CharacteristicElectrophoretic Pattern: Characteristic
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
商品
Methacrylated hydrogels enable 3D cell culture scaffolds and bioprinting using light crosslinking.
Methacrylated hydrogels enable 3D cell culture scaffolds and bioprinting using light crosslinking.
Methacrylated hydrogels enable 3D cell culture scaffolds and bioprinting using light crosslinking.
Methacrylated hydrogels enable 3D cell culture scaffolds and bioprinting using light crosslinking.
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