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Merck

341738

Sigma-Aldrich

磺酰罗丹明 B,酸式

Dye content 95 %

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About This Item

经验公式(希尔记法):
C27H30N2O7S2
CAS号:
分子量:
558.67
EC 号:
MDL编号:
UNSPSC代码:
12352103
PubChem化学物质编号:
NACRES:
NA.23
价格与库存信息目前不能提供

表单

solid

组成

Dye content, 95%

mp

>300 °C (lit.)

λmax

558 nm

SMILES字符串

CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)

InChI key

IOOMXAQUNPWDLL-UHFFFAOYSA-N

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一般描述

磺酰罗丹明 B (SRB) 是一种荧光蛋白染料。 据报告,染料在温和的碱性条件下与三氯乙酸固定细胞的氨基酸残基结合,并在温和的酸性条件下将其自身提取到溶液中。[1]

应用

SRB 对其对活细胞反应性的 pH 敏感性使其可用于药物诱导的细胞毒性和细胞增殖。[1]SRB 实验用于监测活细胞的酶活性。[2]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Samantha N Andrews et al.
Pharmaceutical research, 30(4), 1099-1109 (2012-12-01)
Most methods to increase transdermal drug delivery focus on increasing stratum corneum permeability, without addressing the need to increase permeability of viable epidermis. Here, we assess the hypothesis that viable epidermis offers a significant permeability barrier that becomes rate limiting
Vivek Gupta et al.
PloS one, 8(2), e57136-e57136 (2013-03-14)
Caco-2 monolayers are one of the most widely used in vitro models for prediction of intestinal permeability of therapeutic molecules. However, the conventional Caco-2 monolayer model has several drawbacks including labor-intensive culture process, unphysiological growth conditions, lack of reproducibility and
Zaichang Yang et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 19(3-4), 301-305 (2012-01-14)
Fissistigma cavaleriei (Levl) Rehd (Annonaceae) is used as a folklore medicine for treatment of inflammation, arthritis, and tuberculosis by Miao people in China. In the present study, the antiangiogenic activity of F. cavaleriei was investigated. The chorioallantoic membrane of the

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