V4630

Anti-Vimentin antibody produced in goat

whole antiserum

• Synonyme(s) : Anti-F5H288
• Numéro MDL: MFCD00145922
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: goat
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: whole antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: antigen 58 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: wide range, human
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue; western blot: 1:50 using HS-68 human foreskin cell extract
• Numéro d'accès UniProt: P08670
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... VIM(7431)

Description

Description générale

The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Spécificité

The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

Immunogène

vimentin from cultured human foreskin fibroblasts.

Application

Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis.

Anti-Vimentin antibody produced in goat has been used in following studies:

• immunohistochemistry
• western blotting
• immunocytochemistry
• flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Actions biochimiques/physiologiques

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)