V6630
Monoclonal Anti-Vimentin antibody produced in mouse
clone V9, ascites fluid
Synonyme(s) :
Monoclonal Anti-Vimentin
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
V9, monoclonal
Poids mol.
antigen ~58 kDa
Contient
15 mM sodium azide
Espèces réactives
pig, canine, feline, hamster, rabbit, gerbil, monkey, bovine, chicken, human, horse, rat
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue
western blot: 1:200 using human fibroblasts HS-68
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... VIM(7431)
rat ... Vim(81818)
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved a-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.
Spécificité
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
Immunogène
pig eye lens vimentin.
Application
Monoclonal Anti-Vimentin antibody has been used in western blotting and immunocytochemistry.
Monoclonal Anti-Vimentin antibody produced in mouse has been used as a primary antibody to localize vimentin by immunohistochemistry (IHC) and for immunoprecipitation of vimentin.
Monoclonal Anti-Vimentin antibody produced in mouse has been used:
- in immunofluorescence Analysis
- in western blot
- anti-vimentin antibody is injected into mature oocytes before embryo manipulations to bind endogenous vimentin (VIM)
- in immunocytochemistry
Actions biochimiques/physiologiques
Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.
Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic spread of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.
Vimentin is the major cytoskeletal component in immature glia. It is an intermediate filament protein and serves as a substrate for several caspases. The cleavage of this protein by caspase dismantles intermediate filaments and promotes apoptosis.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Autres remarques
This product can be found as purified product that was produced using cell culture hybridoma product.
V6389 Anti-Vimentin antibody, Mouse monoclonal
clone V9, purified from hybridoma cell culture
V6389 Anti-Vimentin antibody, Mouse monoclonal
clone V9, purified from hybridoma cell culture
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
En option
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Eirik Grønevik et al.
The journal of gene medicine, 5(10), 909-917 (2003-10-09)
Genes encoding non-self proteins may be injected into skeletal muscles in vivo to obtain induction of cellular and humoral immune responses against the encoded antigens (DNA vaccination). Bone marrow derived professional antigen-presenting cells (APCs) play a key role in the
Behzad Gerami-Naini et al.
Endocrinology, 145(4), 1517-1524 (2003-12-20)
Trophoblast differentiation and early placental development are essential for the establishment of pregnancy, yet these critical events are not readily investigated in human pregnancy. We used embryoid bodies (EBs) prepared from human embryonic stem (hES) cells as an in vitro
Kei Miyamoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(17), 7040-7045 (2011-04-13)
Nuclear reprogramming of differentiated cells can be induced by oocyte factors. Despite numerous attempts, these factors and mechanisms responsible for successful reprogramming remain elusive. Here, we identify one such factor, necessary for the development of nuclear transfer embryos, using porcine
D Dahl et al.
European journal of cell biology, 24(2), 191-196 (1981-06-01)
Comparison of cytoskeletal preparations obtained from newborn and adult rat brain showed similar patterns on SDS-PAGE. However, coelectrophoresis of the newborn and adult preparations revealed distinct differences in the mobility of 2 major bands in the molecular weight range of
Bupivacaine-induced regeneration of rat soleus muscle: ultrastructural and immunohistochemical aspects.
Politi PK, et al.
Ultrastructural Pathology, 30(6), 461-469 (2006)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique