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SNC50

Sigma-Aldrich

Mission® Small RNA Purification Kit

1 sufficient for 50 preparations

Synonyme(s) :

microRNA Isolation Kit

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Niveau de qualité

Utilisation

sufficient for 50 preparations

Technique(s)

DNA extraction: suitable

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Description générale

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application

mirPremier® microRNA Isolation Kit has been used to:

  • extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
  • isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
  • extract miRNA, from frozen rat livers for miRNA analysis.

Caractéristiques et avantages

  • Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
  • Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Can extract high-purity miRNA with no detectable large RNA
  • No dangerous organic extractions are involved.

Informations légales

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Rong-Hua Lu et al.
Fish physiology and biochemistry, 46(5), 1665-1677 (2020-05-25)
Hepatic lipid metabolism disorder due to excessive fat accumulation in fish is a significant problem in aquaculture. Studies have shown that grape seed procyanidin extract (GSPE) can regulate fish lipid metabolism and improve fish immunity. However, the mechanism is unclear.
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)

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