SNC10
Mission® Small RNA Purification Kit
1 sufficient for 10 preparations
Synonyme(s) :
microRNA Isolation Kit
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About This Item
Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52
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Description générale
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
mirPremier® microRNA Isolation Kit has been used to:
- prepare small RNA
- enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis
- isolate small RNA from Vigna mungo tissues
- extract miRNAs to detect the expression of miRNA-16
Caractéristiques et avantages
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
Informations légales
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Classification des risques
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Certificats d'analyse (COA)
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Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
Zhang Y, et al.
Genome Biology, 18(1), 105-105 (2017)
Identification of microRNA-mRNA regulatory network associated with oxidative DNA damage in human astrocytes
Nwokwu CD, et al.
ASN Neuro, 14, 17590914221101704-17590914221101704 (2022)
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