RAB0444
Cell-Based ELISA for detecting phospho-STAT3 (pTyr705) in cultured cell lines
96 assays
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About This Item
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
96 assays
Espèces réactives
rat, human, mouse
Technique(s)
ELISA: suitable
capture ELISA: suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... STAT3(6774)
mouse ... STAT3(20848)
rat ... STAT3(25125)
Description générale
The Cell-Based Stat3 (pTyr705) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat3 (pTyr705) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.
The STAT3 (signal transducer and activator of transcription)gene is mapped to human chromosome 17q21.2. Upon phosphorylation, STAT3 translocates to nucleus from cytoplasm, where it mediates a number of biological processes. The protein structure includes the following domains : N-domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain and transcriptional activation domain.
The STAT3 (signal transducer and activator of transcription)gene is mapped to human chromosome 17q21.2. Upon phosphorylation, STAT3 translocates to nucleus from cytoplasm, where it mediates a number of biological processes. The protein structure includes the following domains : N-domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain and transcriptional activation domain.
Immunogène
Stat3 (pTyr705) synthetic phosphopeptide
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Actions biochimiques/physiologiques
Signal transducer and activator of transcription (STAT) proteins are a family of latent cytoplasmic transcription factors involved in cytokine, hormone, and growth factor signal transduction. STAT proteins mediate broadly diverse biological processes, including cell growth, differentiation, apoptosis, fetal development, transformation, inflammation, and immune response. Unlike all other members of the STAT gene family, ablation of STAT3 leads to embryonic lethality and it has been suggested that this protein might represent a primordial STAT protein. It evokes a number of distinct responses in different cells, including induction of an acute-phase response in hepatoma cells, stimulation of proliferation in B lymphocytes, activation of terminal differentiation and growth arrest in monocytes, and maintenance of the pluripotency of embryonic stem cells. Dysregulation of STAT3 signaling has been demonstrated to contribute to malignant cellular transformation. Src kinase-mediated activation of STAT3 has been shown to be essential in prostate and ovarian carcinomas. In head and neck cancers, constitutive STAT3 activity with up-regulated epidermal growth factor receptor (EGFR) signaling plays an important role in malignant proliferation. STAT3 may be a key player in the pathogenesis of diverse human cancers which makes this molecule a prime target for novel therapies.
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Code de la classe de stockage
10 - Combustible liquids
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
The role of IL-6 and STAT3 in inflammation and cancer.
Hodge D R, et al.
European Journal of Cancer, 41(16), 2502-2512 (2005)
Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality.
Takeda K, et al.
Proceedings of the National Academy of Sciences of the USA, 94(8), 3801-3804 (1997)
STATs and gene regulation.
Darnell J E
Science, 277(5332), 1630-1635 (1997)
Crystal structure of unphosphorylated STAT3 core fragment.
Ren Z, et al.
Biochemical and Biophysical Research Communications, 374(1), 1-5 (2008)
STAT3 single-nucleotide polymorphisms and STAT3 mutations associated with hyper-IgE syndrome are not responsible for increased serum IgE serum levels in asthma families.
Wjst M, et al.
European Journal of Human Genetics, 17(3), 352-352 (2009)
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