H5166
Erythropoietin human
EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonyme(s) :
EPO
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About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Essai
≥95% (SDS-PAGE)
Forme
lyophilized powder
Puissance
≤5.0 ng/mL ED50
Qualité
endotoxin tested
Poids mol.
dimer 36 kDa (glycosylated)
Conditionnement
pkg of 10 μg
Conditions de stockage
avoid repeated freeze/thaw cycles
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1 EU/μg
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... EPO(2056)
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Description générale
EPO has been cloned from various species including human, murine, canine, and others. The mature proteins from the various species are highly conserved and exhibit greater than 80% amino acid sequence identity. EPO contains three N-linked glycosylation sites. The glycosylation of erythropoietin is required for the biological activities of erythropoietin in vivo.
Actions biochimiques/physiologiques
Erythropoietin (EPO), produced primarily by the kidney, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells. EPO produced by kidney cells is increased in response to hypoxia or anemia. The biological effects of erythropoietin are mediated by the erythropoietin receptor, which binds EPO with high affinity and is a potent EPO antagonist.
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.
Notes préparatoires
Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Remarque sur l'analyse
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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