R1153

RNase B Glycoprotein Standard from bovine pancreas

≥90% (SDS-PAGE), lyophilized powder

• Synonyme(s) : Ribonuclease B from bovine pancreas, RNase B
• Numéro CAS: 9001-99-4
• Numéro CE : 232-646-6
• Numéro MDL: MFCD00132184
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• product name: RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
• Qualité: Proteomics Grade
• Niveau de qualité: 200
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized powder
• Température de stockage: −20°C
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• Clé InChI: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

Application

RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.

Autres remarques

Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn³⁴ at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man_5-9GlcNAc₂. Due to the heterogeneity in the glycosylation at Asn³⁴, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.

Qualité

RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)