P6516

Poly-L-lysine hydrobromide

suitable for cell culture, Mol wt ≤15,000 by MALLS

• Synonyme(s) : L-Lysine homopolymer hydrobromide
• Numéro CAS: 25988-63-0
• Numéro MDL: MFCD00237230
• Code UNSPSC : 12352209
• ID de substance PubChem : 24898773
• Nomenclature NACRES : NA.26

Propriétés

• product name: Poly-L-lysine hydrobromide, mol wt 4,000-15,000 by viscosity
• Forme: solid
• Niveau de qualité: 200
• Poids mol.: ≤15,000 by MALLS; 4,000-15,000 by viscosity
• Score du produit alternatif plus écologique: old score: 51; new score: 43; Find out more about DOZN™ Scoring
• Caractéristiques du produit alternatif plus écologique: Designing Safer Chemicals; Safer Solvents and Auxiliaries; Reduce Derivatives; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Technique(s): cell culture | mammalian: suitable
• Couleur: white to off-white
• Application(s): cell analysis
• Autre catégorie plus écologique: , Re-engineered
• Température de stockage: −20°C
• Chaîne SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Clé InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Description

Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Designing Safer Chemicals”, "Safer Solvents and Auxiliaries" and “Reduce Derivatives”. Click here to view its DOZN scorecard.

Application

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule. Poly-L-lysine hydrobromide has been used:

• as a component of polyplexes and in DNA condensation experiments
• for coating circular mica disks for atomic force microscopy studies and SuperFrost glass slides for immunofluorescence studies of in HL-60 cells
• as a reference standard for generating calibration curve for the quantification of ε- Poly-L-lysine

Actions biochimiques/physiologiques

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Composants

La poly-L-lysine est un polymère d′acide aminé à charge positive, comportant environ un HBr par résidu de lysine. Le bromhydrate permet de former une poudre cristalline de poly-L-lysine soluble dans l′eau. Une petite quantité de produit peut se trouver dans des structures bêta, car le HBr interfère avec la formation de liaisons d′hydrogène entre les groupes aminés et les groupes carboxyliques ou les parties contenant des atomes N ou O.

Attention

Les solutions stériles sont stables pendant environ 2 ans si conservées entre 2 et 8 °C. Conserver à une température de -20  C en présence d′un déshydratant.

Notes préparatoires

This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)