C7806

Bovine Collagen Type II

from bovine nasal septum, powder, suitable for ELISA assays

• Synonyme(s) : Collagen from bovine
• Numéro CAS: 9007-34-5
• Numéro CE : 232-697-4
• Numéro MDL: MFCD00130825
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.61

Propriétés

• Nom du produit: Collagen from bovine nasal septum, Bornstein and Traub Type II, powder
• Source biologique: bovine nasal septum
• Niveau de qualité: 200
• Forme: powder
• Technique(s): ELISA: suitable
• Solubilité: aqueous acid: soluble
• Numéro d'accès UniProt: P02459
• Température de stockage: 2-8°C
• Informations sur le gène: bovine ... COL2A1(407142)

Description

Description générale

Collagen is a fibrous protein, found in connective tissues, hard tissues, bone, dentin, cementum and also the mineralizing cartilage of the epiphyseal growth plate.[1]

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from bovine nasal septum has been used to coat the plates to estimate the levels of serum anti-cII IgG by enzyme-linked immunosorbent assay (ELISA).[2] It has also been used as a component in the emulsified agent to induce arthritis in rats.[3]

Collagen-type II has been investigated for its importance in haemostasis. Two peptides, CB8 and CB11, are sites of platelet binding and activation on collagen-type 2. Collagen-type 2 has also been used as a model for arthritis in animals, where FR140423, a selective cyclooxygenase (COX)-2 inhibitor, has shown an anti-inflammatory effect without gastric lesions that may occur with treatment via indomethacin.

Collagen Type II is sometimes used to induce an animal model of rheumatoid arthritis. However, under conditions leading to formation of reactive oxygen species, the subjects develop tolerance for auto-antibodies. This is an unusual situation of ROS having a favorable effect.[4]

Actions biochimiques/physiologiques

Collagen is the structural constituent of the dermis, which provides strength and support to the human skin. Variations in collagen participates in the aging process.[5]

Notes préparatoires

An acid soluble collagen soluble in dilute acetic acid at pH 3.0 (5 mg/ml), yielding an opalescent, colorless solution.

Collagen Type II is a triple helix composed of three α1(II) strands.

Prepared by a modification of the pepsin extraction procedure of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)