This product is not sterile as received. Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration as we have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes can be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol. Detailed information can be found in the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
C9301
Chicken Collagen Type II
from chicken sternal cartilage, powder, suitable for cell culture
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Nom du produit
Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
Source biologique
chicken (Sternal cartilage)
Niveau de qualité
Type
Type II (Miller)
Gamme de produits
BioReagent
Forme
powder
Conditionnement
glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg
Concentration
60-80% (biuret)
Technique(s)
cell culture | mammalian: suitable
Couverture de surface
6‑10 μg/cm2
Solubilité
acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
Numéro d'accès UniProt
Spécificité de la liaison
Peptide Source: Fibrinogen
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
chicken ... COL2A1(395069)
Description générale
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is made of three polypeptide chains.[1] Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1[2] chains.[1] Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye.[2] The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.[3]
Application
Collagen from chicken sternal cartilage has been used:
Actions biochimiques/physiologiques
Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.
Caractéristiques et avantages
This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.
Autres remarques
Major component of articular cartilage.
Notes préparatoires
Prepared by a modification of the method of Trentham, D.E., et al.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Is this product sterilized? If not, how do we sterilize it? Thank you!
1 answer-
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May I please know the exact molecular weight of the protein ?
1 answer-
The product C9301 gives 1 major band at approximately 150 KDa in the SDS-PAGE test.
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What is the recommended method for storing stock solutions of item C9301, Collagen from chicken sternal cartilage after reconstitution?
1 answer-
Following reconstitution, store the solution in the refrigerator at 4°C. Stock solutions are stable for up to 6 months at 4°C. It is important to avoid freeze/thaw cycles of the solutions.
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