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EZHS

Sigma-Aldrich

High Sensitivity Human Amyloid β40 and Amyloid β42 ELISA

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Espèces réactives

human

Niveau de qualité

Technique(s)

ELISA: suitable

assay range

inter-assay cv: <10
intra-assay cv: <10
standard curve range: 16 -500 pg/mL

Méthode de détection

colorimetric

Température de stockage

2-8°C

Informations sur le gène

human ... APP(351)

Description générale

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s High Sensitivity Human Amyloid β40 and Amyloid β42 ELISA kits are used for the measurement of Amyloid β40 and Amyloid β42 ELISA in cerebrospinal fluid, cell culture supernatants, primary neurons and plasma in a 96-well format.

Spécificité

The Amyloid β40 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

The Amyloid β42 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-40, Aβ1-43 and Aβ1-44.

Used to detect/quantify: Amyloid β40 & Amyloid β42

Application

This High Sensitivity Human Amyloid β40 & Amyloid β42 ELISA is used to measure & quantify Amyloid β40 & Amyloid β42 levels in Neuroscience research.

These assays require 50 µl of sample and are overnight assays.

Research Category: Neuroscience

Research Sub-Category: Alzheimer′s Disease

Conditionnement

96-well plate

Stockage et stabilité

Components in the kit can be stored up to 2 weeks at 2-8°C

Autres remarques

Contact technical support for information

Clause de non-responsabilité

For research use only. Not for use in diagnostic procedures.

Pictogrammes

CorrosionExclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials


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