HPLC Analysis of Withania Standards on Discovery® C18
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
CONDITIONS
column
Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase
[A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient
5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate
1.5 mL/min
pressure
220 bar (3200 psi)
column temp.
27 °C
detector
UV, 227 nm
injection
20 μL
sample
20 μg/mL in water: acetonitrile (86:14)
Beschreibung
Hinweis zur Analyse
This application demonstrates the suitability of Discovery C18 for the efficient separation of withania standards by the USP monograph.
Rechtliche Hinweise
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany