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HPLC Analysis of Withania Standards on Discovery® C18

HPLC Analysis of Withania Standards on Discovery® C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Discovery® C18 HPLC-Säule

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

Withanosid IV

analytical standard

12-Deoxywithastramonolid

analytical standard

Withanolid A

analytical standard

Withaferin A

analytical standard

CONDITIONS

column

Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)

mobile phase

[A] 1 mM potassium phosphate, monobasic; [B] acetonitrile

gradient

5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min

flow rate

1.5 mL/min

pressure

220 bar (3200 psi)

column temp.

27 °C

detector

UV, 227 nm

injection

20 μL

sample

20 μg/mL in water: acetonitrile (86:14)

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Discovery C18 for the efficient separation of withania standards by the USP monograph.

Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany