HPLC Analysis of Ruelene (Cruformate) Enantiomers on Astec^® CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] acetonitrile; [B] acetic acid; [C] TEA; (100:0.3:0.2, A:B:C)

flow rate

0.6 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

Ruelene (cruformate), 1 mg/mL in acetonitrile