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Merck

SAB4300603

Sigma-Aldrich

Anti-HA-Tag antibody produced in rabbit

affinity isolated antibody

Synonym(e):

Anti-Hemagglutinin-Tag antibody produced in rabbit

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Konzentration

1 mg/mL

Methode(n)

western blot: 1:500-1:1000

Isotyp

IgG

Immunogene Sequenz

(Y-P-Y-D-V-P-D-Y-A)

Anwendung(en)

research pathology

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
This antibody is specific for the HA tag containing fusion proteins and does not detect other proteins.

Immunogen

Peptide sequence (Y-P-Y-D-V-P-D-Y-A)HA-Tag.

Anwendung

Anti-HA-Tag antibody produced in rabbit has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Rabbit polyclonal anti-HA-Tag antibody may be used as an epitope specific reagent for the detection and/or purification of multiple engineered proteins. The specific advantage is that the use of HA-tag sequence in engineered proteins eliminates the requirement to have a protein specific antibody for detection and purification of specific proteins.

Leistungsmerkmale und Vorteile

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physikalische Form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

GATA3 transcription factor abrogates Smad4-mediated fascin overexpression, invadopodium formation and breast cancer cell invasion.
Sun J, et al.
Test, jbc-M113 (2013)
RGS2 modulates the activity and internalization of dopamine D2 receptors in neuroblastoma N2A cells
Luessen DJ, et al.
Neuropharmacology, 110, 297-307 (2016)
Yu Liu et al.
Nature genetics, 49(8), 1211-1218 (2017-07-04)
Genetic alterations that activate NOTCH1 signaling and T cell transcription factors, coupled with inactivation of the INK4/ARF tumor suppressors, are hallmarks of T-lineage acute lymphoblastic leukemia (T-ALL), but detailed genome-wide sequencing of large T-ALL cohorts has not been carried out.
Wei Zhao et al.
Journal of immunology (Baltimore, Md. : 1950), 205(8), 2255-2264 (2020-09-16)
The cytosolic pattern recognition receptor NLRP3 senses host-derived danger signals and certain microbe-derived products in both humans and rodents. NLRP3 activation assembles an inflammasome complex that contains the adapter proteins ASC and caspase-1, whose activation triggers the maturation and release
Reetika Rawat et al.
PLoS genetics, 7(3), e1001350-e1001350 (2011-04-13)
Circadian rhythms provide organisms with an adaptive advantage, allowing them to regulate physiological and developmental events so that they occur at the most appropriate time of day. In plants, as in other eukaryotes, multiple transcriptional feedback loops are central to

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