S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Synonym(e): Basic Red 2, Gossypimin, Safranin T, Safranin Y oder A, Woll-Rot
• Empirische Formel (Hill-System): C₂₀H₁₉ClN₄
• CAS-Nummer: 477-73-6
• Molekulargewicht: 350.84
• Farbindexnummer: 50240
• Beilstein: 3924099
• EG-Nummer: 207-518-8
• MDL-Nummer: MFCD00011759
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24899820
• NACRES: NA.47

Eigenschaften

• product name: Safranin O, certified by the Biological Stain Commission
• Qualität: certified by the Biological Stain Commission
• Qualitätsniveau: 200
• Form: powder
• Zusammensetzung: Dye content, ≥80%
• Methode(n): microbe id | staining: suitable
• Farbe: green to very dark green
• Löslichkeit: H₂O: 1 mg/mL
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• InChIKey: OARRHUQTFTUEOS-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Anwendung

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Eignung

Zertifiziert für die Flemming'sche Dreifachfärbung von Chromosomen, Färbung gramnegativer Bakterien bei der Gramfärbung, Safranin-Echtgrün FCF-Färbung von Pflanzenzellen.

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• H-Sätze: H318
• P-Sätze: P280 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Dam. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Gloves