F7252

Fast Green FCF

Dye content ≥85 %, powder

• Synonym(e): Lebensmittelgrün 3
• Lineare Formel: C₃₇H₃₄N₂O₁₀S₃Na₂
• CAS-Nummer: 2353-45-9
• Molekulargewicht: 808.85
• Farbindexnummer: 42053
• Beilstein: 5718212
• EG-Nummer: 219-091-5
• MDL-Nummer: MFCD00013053
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 57654190
• NACRES: NA.83

Eigenschaften

• product name: Fast Green FCF, Dye content ≥85 %
• Form: powder
• Zusammensetzung: Dye content, ≥85%
• mp (Schmelzpunkt): 290 °C (dec.) (lit.)
• Löslichkeit: H₂O: 1 mg/mL, clear
• SMILES String: [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChIKey: RZSYLLSAWYUBPE-UHFFFAOYSA-L

Beschreibung

Allgemeine Beschreibung

Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.

Anwendung

Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Biochem./physiol. Wirkung

Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Warning
• H-Sätze: H335,H341
• P-Sätze: P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
• Gefahreneinstufungen: Muta. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): 352.4 °F - closed cup
• Flammpunkt (°C): 178 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Gloves