R0884

RNA-Polymerase, T7 aus E. coli HMS 174/pAR1219

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): T7-RNA-Polymerase
• CAS-Nummer: 9014-24-8
• EC-Nummer: 2.7.7.6 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00132197
• UNSPSC-Code: 12352204

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualität: for molecular biology
• Form: buffered aqueous solution
• Mol-Gew.: 98.8 kDa
• Konzentration: 10,000-50,000 U/mL
• UniProt-Hinterlegungsnummer: P00573
• Fremdaktivität: DNase and RNase, none detected
• Lagertemp.: −20°C
• Angaben zum Gen: bacteriophage T7 ... T7p07(1261050)

Beschreibung

Allgemeine Beschreibung

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Komponenten

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Einheitendefinition

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Hinweis zur Analyse

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable