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Wichtige Dokumente
P9290
Proteinase-K–Agarose aus Tritirachium album
lyophilized powder
Synonym(e):
Proteinase K-Agarose
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About This Item
Empfohlene Produkte
Biologische Quelle
microbial (T.album
T. ALBUM)
Qualitätsniveau
Form
lyophilized powder
Kennzeichnungsgrad
30-140 units per g solid
Methode(n)
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
Quellen
1 g swells to ~5 mL
Lagertemp.
−20°C
Anwendung
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Biochem./physiol. Wirkung
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Leistungsmerkmale und Vorteile
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Einheitendefinition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physikalische Form
Lyophilisiertes Pulver stabilisiert mit Lactose
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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