P3076

Platelet-Derived Growth Factor-AA human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): PDGF−AA
• MDL-Nummer: MFCD00081862
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Platelet-Derived Growth Factor-AA human, PDGF-AA, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: lyophilized powder
• Wirksamkeit: 1.0-3.0 ng/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: 28.5 kDa (containing 250 amino acid residues)
• Verpackung: pkg of 10 μg; pkg of 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <1 EU/μg
• UniProt-Hinterlegungsnummer: P04085
• Lagertemp.: −20°C
• Angaben zum Gen: human ... PDGFRA(5154)

Beschreibung

Allgemeine Beschreibung

Platelet-derived growth factor (PDGF) is composed two polypeptide subunits of A and B chain. PDGF is produced by a number of cells including smooth muscle cells, endothelial cells and macrophages. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors.

Biochem./physiol. Wirkung

Cell division and relocalization of pulmonary artery smooth muscle cells and fibroblasts is known to be triggered by platelet-derived growth factor (PDGF). Due to its proliferatory function, it is associated with fibroproliferative disorders such as atherosclerosis, pulmonary arterial hypertension and lung fibrosis. PDGF exists both in homo and heterodimeric forms and stimulates α and β-cell surface receptors. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signalling and is known to promote tumor angiogenesis in vivo.

Sonstige Hinweise

Homodimer of the A chain of human PDGF.

Physikalische Form

Sterile-filtered through a 0.2 μm filter. Lyophilized with 10 mM acetic acid.

Hinweis zur Analyse

The biological activity is measured by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable